Yes, many refrigerant leak detection methods can detect leaks in systems using alternative refrigerants, such as hydrofluoroolefins (HFOs), hydrofluorocarbons (HFCs), and natural refrigerants like CO2 and hydrocarbons. However, the specific detection method and tool may need to be adapted or modified to accommodate the unique properties of the alternative refrigerant. It is essential to consult the manufacturer’s instructions and industry guidelines to ensure the detection method is suitable for the specific alternative refrigerant being used.